File:Microarray and sequencing flow cell.svg

Summary

Description
English: Microarray and sequencing flow cell. Microarrays and RNA-seq rely on image analysis in different ways. In a microarray chip, each spot on a chip is a defined oligonucleotide probe, and fluorescence intensity detects the abundance of a specific sequence (Affymetrix). In a high-throughput sequencing flow cell, spots are sequenced one nucleotide at a time, with the colour at each round indicating the next nucleotide in the sequence (Illumina Hiseq). Other variations of these techniques use more or fewer colour channels.
Date
Source Own work
Author Thomas Shafee
Other versions

Single channel (green) File:Microarray or sequencing chip (green).svg
Dual channel (green + red) version at File:DNA microarray.svg
Quad channel (green + red + Yellow + Blue) version at File:Sequencing flow cell (4 colour).svg

Side-by-side version at File:Microarray_and_sequencing_flow_cell.svg

Licensing

I, the copyright holder of this work, hereby publish it under the following license:
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 4.0 International license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Category:CC-BY-4.0#Microarray%20and%20sequencing%20flow%20cell.svgCategory:Self-published work
Category:Microarray analysis Category:Sequencing techniques Category:High-throughput nucleotide sequencing
Category:CC-BY-4.0 Category:High-throughput nucleotide sequencing Category:Microarray analysis Category:Self-published work Category:Sequencing techniques