English: Clathrin-dependent DI-T internalization. BSC1 cells stably expressing σ2-eGFP (green) were inoculated with DI-T particles, and time-lapse images were acquired at 4 s intervals. A 4.2×4.2 µm2 area of the cell surface is shown as in Figure 2B. The video depicts a single DI-T particle (red) that attaches to the plasma membrane and diffuses slowly. A clathrin-coated pit captures the virus particle when a dim adaptor spot colocalizes with the virus signal. The adaptor fluorescence intensity increases as coated pit assembly proceeds, and particle internalization occurs shortly after the adaptor signal peaks. Disappearance of the adaptor signal signifies clathrin uncoating, and the virus-containing vesicle is then transported toward the cell interior.