English: Dynamics of RBF1 expression during the early infection stages captured by a time-lapse fluorescence imaging method. Inner epidermis of rice leaf sheath was inoculated with Magnaporthe oryzae transformed with RBF1p::GFP, and GFP fluorescence was acquired at 20-min intervals from 18 hpi for 26 h. Z-series of confocal fluorescence images for GFP (indicating RBF1 expression) was stacked. Bar = 20 μm.