File:Filamins-Regulate-Cell-Spreading-and-Initiation-of-Cell-Migration-pone.0007830.s005.ogv

Summary

Description
English: HT1080 cells were transfected with ASB2α. After 20 hours cells were suspended, washed, and seeded onto 5 µg/ml FN-coated plates, 10 minutes after plating unattached cells were washed away and imaging was initiated 1 hour after plating at 1 frame/5 minutes for 5 hours. At the end of the time-lapse recording, cells were fixed and stained for FLNa expression. ASB2α expressing cells were outlined in the last frame and in the immunofluorescence image. Bar  = 20 µm.
Date
Source Movie S4 from Baldassarre M, Razinia Z, Burande C, Lamsoul I, Lutz P, Calderwood D (2009). "Filamins Regulate Cell Spreading and Initiation of Cell Migration". PLOS ONE. DOI:10.1371/journal.pone.0007830. PMID 19915675. PMC: 2773003.
Author Baldassarre M, Razinia Z, Burande C, Lamsoul I, Lutz P, Calderwood D
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Category:Cell adhesion Category:Actin video Category:Videos of HT1080 Category:Contractile proteins Category:Antibodies Category:Jurkat cells Category:Biological models Category:Proteasome endopeptidase complex Category:Media from PLOS ONE Category:Uploaded with Open Access Media Importer Category:Videos from open-access scholarly articles Category:Uploaded with Open Access Media Importer and needing category review Category:Ogv videos Category:Video display resolution 590 x 408
Category:Actin video Category:Antibodies Category:Biological models Category:CC-BY-2.5 Category:Cell adhesion Category:Contractile proteins Category:Jurkat cells Category:Media from PLOS ONE Category:Ogv videos Category:Proteasome endopeptidase complex Category:Uploaded with Open Access Media Importer Category:Uploaded with Open Access Media Importer and needing category review Category:Video display resolution 590 x 408 Category:Videos from open-access scholarly articles Category:Videos of HT1080