English: Movie of the acrosome movements in the wild-type testis in phosphate buffered saline. The time-lapsed images of isolated y w; P [w +mc snky:GFP] testis collected using a 60× (oil) NA 1.42 objective attached to a laser scanning confocal microscope. Each frame was averaged for 2 scans (at around 1 second per scan) and collected every 3 minutes. A total of 20 frames are presented in this movie. The movie runs at two frames per second. The acrosomes of the elongating spermatids (red arrows) were found to move quite well. In comparison, the compacted acrosome bundles were mostly stationary (white arrows). A two-dimensional time projection of these frames is shown in Figure 5(A).