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Summary

Description

English: ER morphology in E. histolytica treated by nitric oxide. Trophozoites expressing GFP-KDEL-FLAG were seeded on glass bottom culture dishes (MatTeck) embedded in a collagen matrix and incubated at 37°C. Immediately before imaging 1 mM SNP was added directly to the top of the matrix that then was covered by a coverslip. The amoebas were then imaged using the Perkin Elmer Spinning Disc Confocal Microscope. All images were acquired using the 40× objective and taking one stack every minute for 1 h using a 0.5 µm Z-step.

Date

2012

Source

Video S2 from Santi-Rocca J, Smith S, Weber C, Pineda E, Hon C, Saavedra E, Olivos-García A, Rousseau S, Dillies M, Coppée J, Guillén N (2012). "Endoplasmic Reticulum Stress-Sensing Mechanism Is Activated in Entamoeba histolytica upon Treatment with Nitric Oxide". PLOS ONE. DOI:10.1371/journal.pone.0031777. PMID 22384074. PMC: 3286455.

Author

Santi-Rocca J, Smith S, Weber C, Pineda E, Hon C, Saavedra E, Olivos-García A, Rousseau S, Dillies M, Coppée J, Guillén N

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Summary