English: Molecular dynamics simulation of proaerolysin F457G. Result of a molecular dynamics simulation of proaerolysin F457G. The secondary structure of every frame, as calculated by the DSSP algorithm, is represented in the cartoon with different colors: yellow is beta sheet; white and cyan are random coil; purple, blue, and red are alpha helix). Mutation F457G, located on the CTP, has a destabilizing effect. Indeed, the CTP loses most of its secondary structure and begins to disconnect from Domain 4. On Domain 4 an unfolding similar to the one observed in the absence of CTP can be detected. The movie was rendered using the VMD software.