English: Rotating three-dimensional reconstructions of LGAC with and without nocodazole treatment, at different phases of CCH stimulation. LGAC expressing YFP-Rab27b are shown, comparing the effects of no treatment to nocodazole treatment on the subapical SV pool within representative acini by acquisition of z-stacks images under different conditions. Control and treated acini were imaged at resting phase prior to any stimulation, imaged after 15 m stimulation with 100 µM CCH, and then also imaged after 15 m CCH stimulation followed by multiple washes with fresh media, and 60 m recovery time without or with additional nocodazole. Results are typical of those from N = 5 separate preparations. Bar represents 5 µm.