File:A-workflow-to-process-3D+time-microscopy-images-of-developing-organisms-and-reconstruct-their-cell-ncomms9674-s11.ogv

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English: Supplementary Movie 10 A single cell is selected at time step 62 (in yellow). Its lineage indicates a single branch with no division. Following its trajectory step by step shows that at time step 71, it actually divides and its color is propagated to only one of its daughters, indicating that the algorithm (SimAnn) did not detect the mitotic event. In the checking mode of the interactive visualization software Mov-IT, the yellow cell at time t70 is linked to its blue daughter at t71. Fixing this link proved being sufficient to recover the complete clonal history of the yellow cell from t62 to the end of the time lapse as it two daughters were otherwise properly tracked.
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Source Video file from Faure E, Savy T, Rizzi B, Melani C, Stašová O, Fabrèges D, Špir R, Hammons M, Čúnderlík R, Recher G, Lombardot B, Duloquin L, Colin I, Kollár J, Desnoulez S, Affaticati P, Maury B, Boyreau A, Nief J, Calvat P, Vernier P, Frain M, Lutfalla G, Kergosien Y, Suret P, Remešíková M, Doursat R, Sarti A, Mikula K, Peyriéras N, Bourgine P (2016). "A workflow to process 3D+time microscopy images of developing organisms and reconstruct their cell lineage". Nature Communications. DOI:10.1038/ncomms9674. PMID 26912388. PMC: 4773431.
Author Faure E, Savy T, Rizzi B, Melani C, Stašová O, Fabrèges D, Špir R, Hammons M, Čúnderlík R, Recher G, Lombardot B, Duloquin L, Colin I, Kollár J, Desnoulez S, Affaticati P, Maury B, Boyreau A, Nief J, Calvat P, Vernier P, Frain M, Lutfalla G, Kergosien Y, Suret P, Remešíková M, Doursat R, Sarti A, Mikula K, Peyriéras N, Bourgine P
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Category:Cell lines Category:Cell proliferation Category:Three-dimensional imaging videos Category:Echinoidea Category:Media from Nature Communications Category:Uploaded with Open Access Media Importer Category:Uploaded with Open Access Media Importer and needing category review Category:Videos from open-access scholarly articles Category:Ogv videos Category:Video display resolution 1152 x 720
Category:CC-BY-4.0 Category:Cell lines Category:Cell proliferation Category:Echinoidea Category:Media from Nature Communications Category:Ogv videos Category:Three-dimensional imaging videos Category:Uploaded with Open Access Media Importer Category:Uploaded with Open Access Media Importer and needing category review Category:Video display resolution 1152 x 720 Category:Videos from open-access scholarly articles