English: Reconstruction of all imaged structures in a high-resolution image. A 2x2 stitched (~ 400 μm × 400 μm × 100 μm) high-resolution image (0.3 μm × 0.3 μm × 0.3 μm per voxel) was used. First, the reconstruction of the large vessels, that is, central vein (CV) (cyan), portal vein (PV) (orange) and bile duct (green) are shown. Then, raw images and the corresponding reconstructed objects of the different structures are shown sequentially: sinusoids (magenta), BC (green), nuclei (random colours) and cells (random colours). Additionally, central lines are shown for the tubular structures. Finally, all segmented structures are shown. This video provides a complete over view of the reconstructed objects in a typical high-resolution image.

DOI:

https://dx.doi.org/10.7554/eLife.11214.022