File:A-New-Zebrafish-Model-of-Oro-Intestinal-Pathogen-Colonization-Reveals-a-Key-Role-for-Adhesion-in-ppat.1002815.s002.ogv

Summary

Description
English: Edwardsiella ictaluri colonizes both sides of the lower jaw of zebrafish larvae. Larva analyzed 3 days post-infection by whole-mount immunofluorescence, using an antibody staining bacteria; fluorescence image (red) superimposed to transmission images (gray). Larva Z-stack taken with a confocal microscope and 40× objective. Ventral view with some lateral tilt, anterior to bottom. The first image of the movies provides a visual help on the top left corner (over the eye) roughly indicating the planes of observation throughout the movie, and a coloured scheme of the cartilages visible in the stack. mc: Meckel's cartilage; pq: palatoquadrate; bh: basihyal; ch: ceratohyal (see Kimmel CB; Miller CT and Moens CB. (2001),Specification and morphogenesis of the zebrafish larval head skeleton. Dev Biol. 15;233(2):239–57.) The yellow line depicts the contour of the fish. Note that figure 3D corresponds to a maximal projection of planes 61 to 75 of the whole stack.
Date
Source Video S1 from Rendueles O, Ferrières L, Frétaud M, Bégaud E, Herbomel P, Levraud J, Ghigo J (2012). "A New Zebrafish Model of Oro-Intestinal Pathogen Colonization Reveals a Key Role for Adhesion in Protection by Probiotic Bacteria". PLOS Pathogens. DOI:10.1371/journal.ppat.1002815. PMID 22911651. PMC: 3406073.
Author Rendueles O, Ferrières L, Frétaud M, Bégaud E, Herbomel P, Levraud J, Ghigo J
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