English: Supplementary Movie 3 Vimentin-Dronpa-M159T. Prior to acquisition, three sample regions (centered at the medial xz-plane of the recorded volume) were deliberately exposed to coincident illumination of the activation and read-out light, which negated the bleaching protection introduced by the 2-photon-activation pulse scheme and consequently resulted in a local signal attenuation. Live-cell recording, 25.0 x 8.0 x 2.0 μm3 (xzy).