File:Accumulative-Difference-Image-Protocol-for-Particle-Tracking-in-Fluorescence-Microscopy-Tested-in-pone.0012216.s012.ogv
Summary
Description |
English: The movies report the reconstruction of the trajectory of one SLN in the cells (target 11 in Fig. 4). The images are reported in direct gray levels (all the other movies are in inverted gray levels). The trajectory lasts for 440 frames and therefore two different color rendering is given here. In this movie one every two frames are inserted in the movie and the whole trajectory is rendered in jet(128) colormap on all the frames of the movie. It is noteworthy that, despite the high density of targets in the image and the several interactions between them (20<frames<110, 180<frame<210; 320<frame<440) the trajectory is fully reconstructed with a low level of ambiguity. |
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Source | Movie S10 from Villa C, Caccia M, Sironi L, D'Alfonso L, Collini M, Rivolta I, Miserocchi G, Gorletta T, Zanoni I, Granucci F, Chirico G (2010). "Accumulative Difference Image Protocol for Particle Tracking in Fluorescence Microscopy Tested in Mouse Lymphonodes". PLOS ONE. DOI:10.1371/journal.pone.0012216. PMID 20808918. PMC: 2923183. | ||
Author | Villa C, Caccia M, Sironi L, D'Alfonso L, Collini M, Rivolta I, Miserocchi G, Gorletta T, Zanoni I, Granucci F, Chirico G | ||
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Category:CC-BY-2.5
Category:Interdisciplinary physics
Category:Intracellular space
Category:Media from PLOS ONE
Category:Molecular imaging
Category:Ogv videos
Category:Time factors
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Category:Video display resolution 180 x 180
Category:Videos from open-access scholarly articles
Category:Videos of digital image processing
Category:Videos of experimental biophysical methods
Category:Videos of fluorescence microscopy